This video explains how to collect samples for Piscirickettsia salmonis tissue imprint examination
- Glass microscope slides
- Clean forceps
- Alcohol and flame
- Slide storage container
- Use clean forceps to gently dab a pea-sized fresh kidney sample onto a sterile gauze pad to remove excess blood/fluid; if there is too much blood/fluid on a sample, it can result in WADDL being unable to evaluate the tissue imprint.
- Use clean forceps (remove debris, dip in alcohol, and flame between each sample) and gauze for each sample to prevent contamination between samples.
- Firmly touch the tissue to two sets of glass microscope slides. Allow tissue imprints to air dry.
- Each microscope slide can fit three tissue imprints total.
- Save one set of slides and ship the other set to WADDL for testing.
- See “Packing guidelines” Resource for information about how to properly package slides for shipping.
- Since this test requires lethal sampling of fish, it is valuable to have backup samples in case slides are damaged or lost during shipping.
- At WADDL, slides are fixed, stained, marked, and then evaluated via light microscopy for P. salmonis.