Faculty

Ryan Driskell

Ryan Driskell

Assistant Professor

rdriskell@vetmed.wsu.edu

Office
Room: BLS 233
Phone: 509-335-5614

 

The core mission of the Driskell Laboratory is to investigate mechanisms that will induce regeneration in skin wounds utilizing fibroblast lineages.

The Story of Scar Formation in Skin and a Way to Achieve Scar-less Wound Repair

Human Skin Immunostained
Picture: Human Skin Immunostained with Epidermal and Fibroblast Markers
The biological process of wound healing in the skin normally results in the formation of a scar, which isn’t a problem if they are small (minor cuts). However, individuals with deep wounds that cover large portions their body would significantly benefit from a way to fully regenerate skin while inhibiting scar formation. This is because current medical procedures do not have the ability to reform hair follicles and sweat glands from deep wounds, such as those that occur during a major surgery. There is also a problem with skin grafts because they only can replace the outer-most layers of cells called the epidermis. The loss of hair follicles and sweat glands in wounded skin inhibits also affects the cosmetic makeup of skin.

 

The story of scar formation in the skin starts with an infliction of a wound, which is essentially a newly created hole in the tissue that becomes filled with many different cell types. This includes migrating blood, mesenchymal cells (fibroblasts), epithelial cells which interact to form new tissue which rebuilds new tissue within the hole. The spatial and temporal interactions during this process is critical for repairing the tissue, which normally results in the formation of a scar. Interestingly, it is possible to modulate the repair process, thereby influencing the quality of repair, by controlling epithelial cells and fibroblasts as they enter the blood clot.

We have recently discovered that, by controlling the nature of a fibroblasts before and after wounding in the skin, we can influence hair regeneration in the wound, which represents an essential part of skin regeneration (Driskell e al. 2013 – Nature).  In addition, we found that the reason why scars normally lack hair is because adult skin lacks fibroblast subtypes that support hair follicle development. Furthermore, we have also found why young skin heals better than older skin. This is because the critical cells required for hair follicle neogenesis can be found in normal young (neonatal) dermis of mice or can be induced to replicate by increasing Wnt signalling in the epidermis before wounding (Driskell et al. 2013; Rognoni et al. 2016).  Despite these advances, our findings revealed a need to investigate the mechanisms that control the type of hair follicle regeneration in the wound and its ability to function.

Select Publications
  • Salz L, and Driskell RR. (2017) The Sox2: GFP+/- knock in mouse model does not faithfully recapitulate Sox2 expression in skin. Exp Dermatol. doi: 10.1111/exd.13396.
  • Salz L, and Driskell RR. (2017) A novel processing and imaging protocol for thick three-dimensional cross-sections of skin called “Horizontal Whole Mount”. Journal of Visualized Experiments In Press
  • Rognoni E, Gomez, Pisco AO, Rawlins EL, Watt FM, and Driskell RR. (2016) Inhibition of b-catenin signaling in dermal fibroblasts enhances hair follicle regeneration during wound healing. Development 143:2522-2535; doi 10.1242/dev.131797.
  • Mastrogiannaki M, Lichtenberger BM, Reimer A, Collins CA, Driskell RR, and Watt FM. (2016) b-catenin stabilization in skin fibroblasts causes fibrotic lesions by preventing adipocyte differentiation of the reticular dermis. J. Invest. Dermatol. 136(6):1130-42. doi: 10.1016/j.jid2016.01.036.
  • Kaushal G, Rognoni E, Lichtenberger BM, Driskell RR, Kretzschmar K, Hoste E, and Watt FM. (2015) Fate of Prominin-1 expressing dermal papilla cells during homeostasis, wound healing and Wnt activation. J. Invest. Dermatol. 135(12):2926-34. Doi: 10.1038/jid.2015.319
  • Kretzschmar K, Weber C, Driskell RR, Calonje E, Watt FM. (2016) Compartmentalized epidermal activation of beta-catenin differentially affects lineage reprogramming and underlies tumor heterogeneity. Cell Rep. 12;14(2):269-81
  • Driskell RR, Watt FM. (2015) Understanding fibroblast heterogeneity in the skin. Trends Cell Biol. 25(2): 92-9.
  • Driskell RR, Jahoda CA, Chuong CM, Watt FM, and Horsley V. (2014) Defining dermal adipose tissue.  Exp Dermatol. 23(9):629-31. doi: 10.1111/exd.12450.
  • Driskell RR, Lichtenberger BM, Hoste E, Kretzschmar K, Simons B, Charalambous M, Ferron SR, Herault G, Pavlovic G, Ferguson-Smith AC, and Watt FM. (2013) Distinct fibroblast lineages determine dermal architecture during skin development and repair. Nature. 12;504(7479):277-81. doi: 10.1038/nature12783.
  • Lesko M*, Driskell RR*, Kretzschmar K, Goldie S, Watt FM. (2013) Sox2 modulates the function of two distinct cell lineages in mouse skin. Dev Biol. 1;382(1):15-26. doi: 10.1016/j.ydbio.2013.08.004. Epub 2013 Aug 13.
  • Driskell RR*, Juneja VR*, Connelly JT, Kretzschmar K, Tan DW, Watt FM. (2011) Clonal growth of dermal papilla cells reveals intrinsic differences between Sox2-Positive and –Negative cells in vitro and in vivo. J. Invest. Dermatol. Doi:10.1038/jid2011.428 [Epub ahead of print]
  • Arwert EN, Mentink RA, Driskell RR, Hoste E, Goldie SJ, Quist S, Watt FM. (2011) Upregulation of CD26 expression in epithelial cells and stromal cells during wound-induced skin tumor formation. Oncogene. doi: 10.1038/onc.2011.293 [Epub ahead of print]
  • Driskell, R.R., Clavel C., Rendl, M., Watt F.M. (2011) Hair follicle dermal papilla cells at a glance. Journal of Cell Science 15; 124 (pt8) 1179-1182.
  • *Jensen, K.B. *Driskell, R.R Watt, F.M. (2010) Assaying proliferation and differentiation    capacity of stem cells using disaggregated adult mouse epidermis. Nature Protocols. 5 (5): 898-911.
  • Driskell, R.R., Giangreco, A., Jensen, K.B., Mulder, K.W., and Watt, F.M. (2009) Sox2-positive dermal papilla cells specify hair follicle type in mammalian epidermis. Development 136 (16): 2815-23.
  • Watt F.M., Driskell R.R. (2010) The therapeutic potential of stem cells. Phil Trans Royal Society 365:1537 pg155-163
  • Driskell, R.R. Goodheart, M. Neff, T., Liu, X., Moothart C.M., and Engelhardt, J.F. (2007) Wnt3a Regulates Lef-1 Expression During Airway Submucosal Gland Morphogenesis. Dev Bio. 1:305(3): 90-102
  • Liu, X., Driskell, R.R. and Engelhardt J.F. (2006) Stem cells in the lung. Methods Enzymol. 419;285-321.
  • Liu, X., Driskell, R.R. and Engelhardt, J.F. (2004) Airway Glandular Development and Stem Cells. Current Topics in Developmental Biology Vol. 6, Elsevier, Inc. 64:33-56
  • Driskell, R.R., Liu, X., Luo, M., Filali, M., Abbott, D., Cheng, N., Moothart, C., Sigmund, C.D., and Engelhardt, J.F. (2004) Wnt-Responsive Element Controls Lef-1 Promoter Expression During Submucosal Gland Morphogenesis. American Journal of Physiology-LCMP. Am. J. Physiol. Lung Cell Mol. Physiol. 287:L752-63.
  • Liu, X., Driskell, R.R., Luo, M., Abbot, D., Filali, M., Cheng, N., Sigmund, C.D., and Engelhardt, J.F. (2004) Characterization of Lef-1 Promoter Segments that Facilitate Inductive Developmental Expression in Skin. J. Invest. Dermatol. 123(2):264-74.
  • Driskell, R.R., and Engelhardt, J.F. (2004) Stem Cells in the Adult Lung. Handbook of Stem Cells
  • Liu, X., Yan, Z., Luo, M., Zak, R., Li, Z., Driskell, R.R., Huang, Y., Tran, N., and Engelhardt, J.F. (2004) Targeted correction of single-base-pair mutations with adeno-associated virus vectors under nonselective conditions. J. Virol. 78:4165-75.
  • Driskell, R.A., and Engelhardt, J.F. (2003) Current status of gene therapy for inherited lung diseases. Annu. Rev. Physiol. 65:585-612.