Purpose: Immunohistochemistry involves the detection of specific chemical substances (infectious agents or cellular markers) within tissue by the use of derived antibodies to the substances. Antibodies (polyclonal or monoclonal) are applied to tissue sections and allowed to bind to their corresponding antigen. A detection system is then employed to identify the location of these antibodies using amplified marker molecules that can be visually recorded. These identifiable substrates offer a documentation of localization that can be seen through the microscope at the cellular level. In this way, individual cells or groups of cells can be monitored for the presence of the specific antigen.
The techniques for detection are numerous, but the overall technology is essentially the same. WADDL currently utilizes multiple detection systems including the streptavidin-biotin complex immunoperoxidase detection method, an alkaline phosphatase Fast Red/Luxol detection method, and peroxidase anti-peroxidase method depending on the specific test. The results are interpreted and reported by the pathologist.
Samples: Formalin-fixed tissues or formalin-fixed paraffin-embedded tissues
Positive and negative control slides provided with each ordered test:
- Positive control: Positive tissue incubated with positive antibody
- Negative control: Test tissue incubated with matched concentration of irrelevant antibody
Ordering Information: Include animal species and tissue type for each case
Immunostain: Chromogen for all IHC assays depends upon the test run and include both amino-ethyl-carbazole (AEC), which is red/brown and UltraView Red, which is bright magenta.. Counterstain for all IHC assays is Mayer's hematoxylin, which is blue.
|Test||Specimen||Shipping Container||Max. Turnaround||Comments|
|Tissues in 10% Formalin OR Formalin fixed paraffin-embedded tissue.||Leakproof jar or Styrofoam mailer|
7 working days
|Results interpreted as “Detected” or “Not Detected” and reported separately from pathology report.|
|Tumor Markers||Tissues in 10% Formalin OR Formalin fixed paraffin-embedded tissue.||Leakproof jar or Styrofoam mailer|
7 working days
|Results interpreted as “positive” or “negative” and reported as part of pathology report|
All cases are charged a $10.00 accession fee. Available procedures and prices can be accessed in the WADDL Web Test Search Tool. Type in "IHC" and click on Search.
|Infectious Agents||Clinical Disease||Comments|
|Bovine Herpesvirus-1 (IBR)||Abortion, Respiratory|
|Bovine Respiratory Syncytial Virus||Respiratory|
|Bovine Viral Diarrhea Virus||Abortion, Enteric, Respiratory, Systemic|
|Bovine Viral Diarrhea - Ear Notch||BVD Persistent infection (PI)||BVD Antigen ELISA preferred|
|Canine Distemper Virus|
|Coxiella burnetii||Abortion||Reportable disease|
|Equine Herpesvirus-1||Abortion, Respiratory|
|Feline Coronavirus (FIP)||Feline Infectious Peritonitis|
|Leptospira spp.||Abortion, Systemic|
|Listeria monocytogenes||Abortion, Neurologic|
|Tumor Markers||Neoplasm||Cell Specificity|
|Cytokeratin (Pan)||Carcinoma||Epithelial cell|
|Factor VIII||Hemangiosarcoma||Endothelial cell|
|Glial fibrillary acidic protein (GFAP)|
|Lambda light chain||Plasmacytoma||Plasma cell|
|Muscle Actin||Leiomyosarcoma, Rhabdomyosarcoma||Skeletal muscle myocyte, Smooth muscle myocyte|