Katie Snekvik, Microbiologist, reading cell culture plates for virus isolation. Regulations require that fish must be disease free for national and international export.
WADDL is USDA certified for export testing.
The Aquatic Health Section of WADDL provides health screening and diagnostic testing for marine and fresh water fish, including ornamental species.
WADDL is fully accredited by the AAVLD and one of the few NAHLN laboratories to offer full service aquatic health testing. Additionally, we are USDA approved for testing live fish products for export. We have two AFS and Canadian Health Inspectors on staff who are available to assist with testing for interstate and international movement of live fish products.
Along with our ACVP board-certified Anatomic Pathologist, Dr. Kevin Snekvik, who specializes in Aquatic Animals, the Aquatic Section has a highly educated and experienced staff consisting of 6 microbiologists and a manager.
The Aquatic Health Section of WADDL offers a wide variety of tests for health screening/inspection/certification and diagnostic purposes, allowing our clients to be able to complete all testing in one place, i.e. virology, microbiology, parasitology, pathology, & molecular diagnostics.
Aquatic Health Inspection Testing
Aquatic animal health testing is performed in accordance with the guidelines outlined in both the OIE Manual of Diagnostic Tests for Aquatic Animals and the AFS-USFWS Blue Book.
Turn around time for all health inspections is 29 days based on the requirements set forth in both manuals for virology testing. All cases are charged an $10.00 accession fee.
Two to four weeks advanced notice for scheduling purposes is strongly recommended on all health inspection samplings. Call Jim Thompson at (509) 335-6656 or send an email to firstname.lastname@example.org.All cases are charged a $10.00 accession fee. Available procedures and prices can be accessed in the WADDL Web Test Search Tool. Type in "aqua" and click on Search.
|Culture Identification of specific bacteria (per fish)||Kidney streaks on TSA, BHI or Blood Agar plates||Maintain samples below 100C|
|Aerobic culture, contaminated plate surcharge (per tissue)|
|Renibacterium salmoninarum (BKD)|
Culture (per fish)
BKD FA (per test)
BKD ELISA (per test)
|Fresh Kidney or Ovarian Fluid||Maintain samples below 100C|
|Piscirickettsia salmonis Direct tissue touch exam|
|Fresh Kidney||Maintain samples below 100C|
digest, small to medium sized fish (per test)
|Head, skull section, gill arches||Maintain samples below 100C|
|M. cerebralis digest, large fish (per test)||Head, skull section, gill arches||Maintain samples below 100C|
|Ceratomyxa shasta by intestinal smear (per test)||Intestinal Mucosa|
|Amoebic Gill Disease|
|Bothriocephalus acheilognathi (Asian tapeworm) (per test)||Anterior 1/3 of intestine||Maintain samples below 100C|
|Viral Culture (per test per cell line)|
Viral pathogens routinely tested for include:
Epizootic hematopoietic necrosis virus
|Maintain samples below 100C|
|VHSV testing only (EPC cells)|
|Cell lines in active culture include:|
Epithelioma Papulosum Cyprini (EPC)
Chinook Salmon Embryo (CHSE-214)
Salmon Head Kidney (SHK-1)
Fat Head Minnow (FHM)
Bluegill Fry (BF-2)
Contact lab for additional pathogens and/or
Aquatic Diagnostic TestingAll cases are charged an $10.00 accession fee. Available procedures and prices can be accessed in the WADDL Web Test Search Tool. Type in "aqua" and click on Search.
|Aerobic culture (per tissue)||Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates||Maintain samples below 100C|
|Anerobic culture (per tissue)||Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar plates||Maintain samples below 100C|
|Fungal culture (genus)|
|Viral isolation in cell culture (up to 4 cell lines)|
CHSE-214, EPC, SHK, FHM, BF-2
|Fresh kidney/spleen/pyloric caeca/gill filliment||Maintain samples below 100C|
|Each additional cell line|
|External parasite screen on fresh tissues (per fish)||live fish|
|Necropsy (includes gill wet prep/mucus-skin scrape)|
Fish 1 - 3
Each additional fish
|Histopathology-Tissues from up to 3 fish|
Each additional fish
|Formalin fixed whole fish, tissues|
|External parasite screen by Histopathology (per slide)||Formalin fixed whole fish, tissues|
|Myxobolus cerebralis histopathology (per slide)||Formalin fixed head, skull section, gills|
|Sample collection at WADDL||Whole fish|
|Mini sampling (per fish)|
|Standard sampling (per fish)|
|Sampling kit (plus freight)|
|Molecular Diagnostics - organism identification by PCR||tissue, culture media|
|Infectious pancreatic necrosis virus|
|Infectious Salmon Anemia virus *|
|Infectious hematopoietic necrosis virus (IHNV)|
|Fungal identification by universal primers and sequencing|
|Mycobacterium spp. Identification by universal primers and sequencing|
|Renibacterium salmoninarum (BKD) Nested|
|Spring viremia of carp virus (SVCV)|
|Viral hemorragic septicmia virus|
|*USDA-APHIS Program testing only|