Aquatic Health

Aquatic Health
Katie Snekvik, Microbiologist, reading cell culture plates for virus isolation. Regulations require that fish must be disease free for national and international export.
WADDL is USDA certified for export testing.

Aquatic Health

The Aquatic Health Section of WADDL provides health screening and diagnostic testing for marine and fresh water fish, including ornamental species.

WADDL is fully accredited by the AAVLD and one of the few NAHLN laboratories to offer full service aquatic health testing.  Additionally, we are USDA approved for testing live fish products for export.  We have two AFS and Canadian Health Inspectors on staff who are available to assist with testing for interstate and international movement of live fish products.

Along with our ACVP board-certified Anatomic Pathologist, Dr. Kevin Snekvik, who specializes in Aquatic Animals, the Aquatic Section has a highly educated and experienced staff consisting of 6 microbiologists and a manager. 

The Aquatic Health Section of WADDL offers a wide variety of tests for health screening/inspection/certification and diagnostic purposes, allowing our clients to be able to complete all testing in one place, i.e. virology, microbiology, parasitology, pathology, & molecular diagnostics.

Aquatic Health Inspection Testing


Aquatic animal health testing is performed in accordance with the guidelines outlined in both the OIE Manual of Diagnostic Tests for Aquatic Animals and the AFS-USFWS Blue Book.

Turn around time for all health inspections is 29 days based on the requirements set forth in both manuals for virology testing.  All cases are charged an $10.00 accession fee.

Two to four weeks advanced notice for scheduling purposes is strongly recommended on all health inspection samplings.  Call Jim Thompson at (509) 335-6656 or send an email to thompsj@vetmed.wsu.edu.

All cases are charged a $10.00 accession fee.  Available procedures and prices can be accessed in the WADDL Web Test Search Tool.  Type in "aqua" and click on Search.
TestSpecimen TypeComments

Culture Identification of specific bacteria (per fish)Kidney streaks on TSA, BHI or Blood Agar platesMaintain samples below 100C
Aerobic culture, contaminated plate surcharge (per tissue)
Renibacterium salmoninarum (BKD)
Culture (per fish)
BKD FA (per test)
BKD ELISA (per test)
Fresh Kidney or Ovarian FluidMaintain samples below 100C
Piscirickettsia salmonis Direct tissue touch exam
Culture
Touch Exam
Fresh KidneyMaintain samples below 100C
M. cerebralis
digest, small to medium sized fish (per test)
Head, skull section, gill archesMaintain samples below 100C
M. cerebralis digest, large fish (per test)Head, skull section, gill archesMaintain samples below 100C
Ceratomyxa shasta by intestinal smear (per test)Intestinal Mucosa
Amoebic Gill Disease
Bothriocephalus acheilognathi (Asian tapeworm) (per test)Anterior 1/3 of intestineMaintain samples below 100C
Viral Culture (per test per cell line)

Viral pathogens routinely tested for include:

Epizootic hematopoietic necrosis virus
Infectious hematopoietic necrosis virus
Infectious pancreatic necrosis virus
Oncorhynchus masou virus
Viral hemorrhagic septicemia virus
Infectious salmon anemia virus
Spring Viremia of Carp
Largemouth bass virus

Maintain samples below 100C
VHSV testing only (EPC cells)
Cell lines in active culture include:

Epithelioma Papulosum Cyprini (EPC)
Chinook Salmon Embryo (CHSE-214)
Salmon Head Kidney (SHK-1)
Fat Head Minnow (FHM)
Bluegill Fry (BF-2)

Contact lab for additional pathogens and/or
cell lines

Aquatic Diagnostic Testing

All cases are charged an $10.00 accession fee. Available procedures and prices can be accessed in the WADDL Web Test Search Tool.  Type in "aqua" and click on Search.
TestSpecimen TypeComments
Bacteriology
Aerobic culture (per tissue)Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar platesMaintain samples below 100C
Anerobic culture (per tissue)Fresh kidney, spleen, liver, brain; bacterial swab; streaked TSA, BHI or Blood Agar platesMaintain samples below 100C
Antimicrobial susceptibility
Fungal culture (genus)
Mycobacterium (genus)
Virology
Viral isolation in cell culture (up to 4 cell lines)
CHSE-214, EPC, SHK, FHM, BF-2
Fresh kidney/spleen/pyloric caeca/gill fillimentMaintain samples below 100C
Each additional cell line
Parasitology
External parasite screen on fresh tissues (per fish)live fish
Pathology
Necropsy (includes gill wet prep/mucus-skin scrape)
Fish 1 - 3
Each additional fish
live fish
Histopathology-Tissues from up to 3 fish
Each additional fish
Formalin fixed whole fish, tissues
External parasite screen by Histopathology (per slide)Formalin fixed whole fish, tissues
Myxobolus cerebralis histopathology (per slide)Formalin fixed head, skull section, gills
Sample collection at WADDLWhole fish
Mini sampling (per fish)
Standard sampling (per fish)
Sampling kit (plus freight)
Molecular Diagnostics - organism identification by PCR
tissue, culture media
Flavobacterium psychrophilum
Infectious pancreatic necrosis virus
Infectious Salmon Anemia virus *
Infectious hematopoietic necrosis virus (IHNV)
Koi herpesvirus
Fungal identification by universal primers and sequencing
Mycobacterium spp. Identification by universal primers and sequencing
Myxobolus cerebralis
Renibacterium salmoninarum (BKD) Nested
Spring viremia of carp virus (SVCV)
Viral hemorragic septicmia virus
Yersinia ruckeri
Direct PCR/sequencing
Cloning/sequencing
*USDA-APHIS Program testing only